© Agilent Technologies, Inc., 2015, 2017

Published in USA, June 1, 2017

Publication Number 5991-5211EN

DNA analysis during the NGS

workflow

The Agilent 4200 TapeStation system

and the DNA ScreenTape assays can be

used at several steps of the Next gener-

ation Sequencing (NGS) workflow such

as shearing of genomic DNA and library

amplification to ensure DNA library and

sample quality. The figure shows the

analysis of sheared genomic DNA for an

NGS library preparation workflow with

the D5000 ScreenTape assay.

Agilent D5000 ScreenTape applications

Learn more

www.agilent.com/genomics/tapestation

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www.agilent.com/chem/contactus

For Research Use Only.

Not for use in diagnostic procedures.

This information is subject to change without

notice.

1

Signal/noise ratio > 3 for a single peak

2

Determined using D5000/High Sensitivity D5000 ladder as sample

3

Measured against 2200 TapeStation system

Analytical

Specifications

Agilent

D5000 ScreenTape Assay

Agilent High Sensitivity

D5000 ScreenTape Assay

Sizing range

100 bp – 5,000 bp

100 bp – 5,000 bp

Typical resolution

400 – 5,000 bp: 15%

400 – 5,000 bp: 15%

0.1 ng/µL

5 pg/µL

5% CV

10% CV

± 10%

± 15%

Quantitative precision

0.1 – 1 ng/µL: 15% CV

1 – 50 ng/µL: 10% CV

15% CV

± 20%

± 25%

Quantitative range

0.1 – 50 ng/µL

10 – 1,000 pg/µL

Maximum sample buffer

strength

250 mM KCl

250 mM Tris-HCl

125 mM NaCl

50 mM Acetate

25 mM MgCl

2

25 mM BSA

25 mM Guanidine-HCl

25 mM KCl

25 mM Tris-HCl

12.5 mM NaCl

5 mM Acetate

2.5 mM MgCl

2

2.5 mM BSA

2.5 mM Guanidine-HCl

Physical

Specifications

Agilent

D5000 ScreenTape Assay

Agilent High Sensitivity

D5000 ScreenTape Assay

Analysis time

15 samples < 25 minutes

96 samples < 135 minutes

15 samples < 20 minutes

96 samples < 120 minutes

Samples per consumable

15

15

Sample volume required

1 µL

2 µL

Kit stability

4 months

4 months

Kit size

105 samples

105 samples